Journal: Acta pharmacologica Sinica

Article Title: Synthesis of the novel PARP-1 inhibitor AG-690/11026014 and its protective effects on angiotensin II-induced mouse cardiac remodeling.

doi: 10.1038/aps.2016.159

Figure Lengend Snippet: Figure 2. 6014 attenuated the enlargement of the heart size, cardiomyocytes size and prevented the accumulation of collagen. (A) Representative diagram of mice hearts under different treatment. (B) Representative images diagram of H&E staining of cardiomyocytes. (C) Representative images of Masson’s staining of total collagen in tissue sections of heart. (D) Statistical results of HW/BW. (E) Statistical results of LVW/BW. (F) Statistical areas of total collagen production versus areas of whole tissue. Data were expressed as mean±SEM from three independent experiments. **P<0.01 vs Sham. #P<0.05, ##P<0.01 vs Ang II.

Article Snippet: Anti-PARP-1, anticollagen I and anti-collagen III antibodies were provided by Proteintech Group (Chicago, IL, USA).

Techniques: Staining

Journal: Acta pharmacologica Sinica

Article Title: Synthesis of the novel PARP-1 inhibitor AG-690/11026014 and its protective effects on angiotensin II-induced mouse cardiac remodeling.

doi: 10.1038/aps.2016.159

Figure Lengend Snippet: Figure 3. Effects of 6014 on the expression of hypertrophy marker proteins and fibrosis marker proteins. Protein expression of ANF (A), BNP (B), β-MHC (C), FN (D), collagen I (E) and collagen III (F) were measured by Western blot. Data were expressed as mean±SEM from duplicates in three independent experiments. **P<0.01 vs Sham. #P<0.05, ##P<0.01 vs Ang II.

Article Snippet: Anti-PARP-1, anticollagen I and anti-collagen III antibodies were provided by Proteintech Group (Chicago, IL, USA).

Techniques: Expressing, Marker, Western Blot

Journal: Acta pharmacologica Sinica

Article Title: Synthesis of the novel PARP-1 inhibitor AG-690/11026014 and its protective effects on angiotensin II-induced mouse cardiac remodeling.

doi: 10.1038/aps.2016.159

Figure Lengend Snippet: Figure 4. Effects of 6014 on the expression and enzymatic activity of PARP-1. The expression of PARP-1 in nucleus (A) and whole cell (B) as well as PARylated proteins in whole cell (C). Data were expressed as mean±SEM from duplicates in three independent experiments. *P<0.05, **P<0.01 vs Sham. ##P<0.01 vs Ang II.

Article Snippet: Anti-PARP-1, anticollagen I and anti-collagen III antibodies were provided by Proteintech Group (Chicago, IL, USA).

Techniques: Expressing, Activity Assay

Journal: Acta pharmacologica Sinica

Article Title: Synthesis of the novel PARP-1 inhibitor AG-690/11026014 and its protective effects on angiotensin II-induced mouse cardiac remodeling.

doi: 10.1038/aps.2016.159

Figure Lengend Snippet: Figure 6. Effects of 6014 on the regulation and activity of SIRT-1. NAD+ content analysis (A) and SIRT-1 activity (B) were analyzed. Protein expression of SIRT-1 were measured by Western blot (C). The interaction between SIRT-1 and PARP-1 (D) and the PARylation of SIRT-1 (E) were measured by co-IP assay. Data were expressed as mean±SEM from three independent experiments. *P<0.05, **P<0.01 vs Sham. #P<0.05, ##P<0.01 vs Ang II.

Article Snippet: Anti-PARP-1, anticollagen I and anti-collagen III antibodies were provided by Proteintech Group (Chicago, IL, USA).

Techniques: Activity Assay, Expressing, Western Blot, Co-Immunoprecipitation Assay

Journal: Cell metabolism

Article Title: Multiplexed in Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes

doi: 10.1016/j.cmet.2019.01.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 169Tm-Collagen Type 1 , Fluidigm , Cat#3169023D.

Techniques: Software